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상품명 | [MBCELL] Baird Parker Agar (500g) (98289) |
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판매가 | ₩180,400 (VAT 포함) |
수량 | |
적립금 | 1,800원 |
★ 기타 | MBCELL 주문시 반품 및 교환 불가 (본사 지침사항) |
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*** 네이버페이 구매 시 제주도 지역 추가 배송비 안내 ***
네이버페이 구매 시에는 제주도지역 추가 배송비가 자동적용이 안되기 때문에 추가 배송비를 별도로 입금을 해주셔야 합니다. 기업은행 052-072431-01-014 예금주: ㈜비엔에프코리아, 추가배송비 3,000원 입금부탁드리며, 입금확인 후 출고진행되오니 이점 양해부탁드립니다. ★구매자명으로 입금해주세요~Medium used for the isolation and cultivation of Staphylococcus aureus.
*Equally use with MFDS (MB-B1004K) and KFCC (MB-B1004C).
CONTENTS (Liter) | Tryptone | 10.0 g |
Beef Extract | 5.0 g | |
Yeast Extract | 1.0 g | |
Glycine | 12.0 g | |
Sodium Pyruvate | 10.0 g | |
Lithium Chloride | 5.0 g | |
Agar | 20.0 g | |
Final pH = 7.2 ± 0.2 at 25°C |
Suspend 63.0 G of powder in 950 mL of distilled or deionized water. Heat to boiling until completely dissolved. Sterilize by autoclave at 121°C for 15 minutes. Cool to 45 - 50°C in water bath. Aseptically add 50 ml of Egg Yolk Tellurite Emulsion (MB-E1863) for observed lecithinase reaction or add 10 vials of R.P.F. supplement (MBR1857) for directly observed coagulase reaction to the medium. Mix well. Pour into petri dishes.
1 vial contents (each vial is sufficient for 100 mL of medium) | |
Bovine Fibrinogen | 0.5g |
Rabbit Plasma | 2.5g |
Trypsin Inhibitor | 0.0025g |
Potassium Tellurite | 0.0025g |
Baird Parker Agar is a medium used for the isolation and cultivation of Staphylococcus aureus. Tryptone and beef extract are the carbon and nitrogen sources. Yeast extract provides vitamin B complex that stimulate bacterial growth. Glycine and sodium pyruvate stimulate the growth of Staphylococci. Lithium chloride is the selective agent. Agar is the solidifying agent. Egg yolk tellurite emulsion is source of lecithin that helps observe the lecithinase reaction of coagulase positive Staphylococci. Coagulase reaction is observed with opaque halo when use only R.P.F supplement to the medium.
Inoculate the specimen using a sterile loop to the medium. Incubate at 35 ± 2°C for 18 - 24 hours up to 48 hours. Refer appropriate references for recommended test procedure.
Dehydrated medium
Appearance: free-flowing, homogeneous
Color: light beige
Prepared medium
Appearance: opaque
Color: yellow
Incubation conditions: 35 ± 2°C / 18 - 24 hours up to 48 hours
Microorganism | ATCC | Inoculum CFU | Growth | Characteristics | Lecithinase reaction | Coagulase reaction |
Staphylococcus aureus | 25923 | 50-100 | good | black colonies | + | + |
Proteus mirabilis | 25933 | 50-100 | partially inhibited | brown colonies | - | - |
Bacillus subtilis | 9466 | ≥10³ | partially inhibited | brown colonies | - | - |
Escherichia coli | 25922 | ≥10³ | partially inhibited | - | - | - |
The powder is very hygroscopic. Store the powder at room temperature, in a dry environment, in its original container tightly closed and use it before the expiry date on the label. Store prepared medium at 2 - 8°C.
1. Baird Parker, A.C.. (1962). An. J. Appl. Bacteriol. 25:12-19
2. Baird Parker. A.C. (1969) Isolation methods for microbiologists. Shapthon,. & Gould ed. London: Academic Press
3. ISO 6888-1:1999. Part 1: Technique using Baird-Parker agar medium.
4. Refer to the MFDS and KFCC.
Cat. No : MB-B1004 Baird Parker Agar | 500 G |
( 출처 : 기산바이오 )
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