스토어 정보 |
---|
상품명 | [MBCELL] Tetrathionate Broth (500g) (01142) |
---|---|
판매가 | ₩91,300 (VAT 포함) |
수량 | |
적립금 | 910원 |
★ 기타 | MBCELL 주문시 반품 및 교환 불가 (본사 지침사항) |
구매방법 | |
---|---|
배송주기
정기배송 할인 save
|
상품 정보 | 가격 | 삭제 |
---|
할인가가 적용된 최종 결제예정금액은 주문 시 확인할 수 있습니다.
*** 네이버페이 구매 시 제주도 지역 추가 배송비 안내 ***
네이버페이 구매 시에는 제주도지역 추가 배송비가 자동적용이 안되기 때문에 추가 배송비를 별도로 입금을 해주셔야 합니다. 기업은행 052-072431-01-014 예금주: ㈜비엔에프코리아, 추가배송비 3,000원 입금부탁드리며, 입금확인 후 출고진행되오니 이점 양해부탁드립니다. ★구매자명으로 입금해주세요~Medium used for the isolation and cultivation of Salmonella spp.
*Equally use with MFDS(MB-T1183K).
CONTENTS (Liter) | Polypeptone | 5.0 g |
Bile Salts | 1.0 g | |
Calcium Carbonate | 10.0 g | |
Sodium Thiosulfate | 10.0 g | |
Final pH = 8.0 ± 0.2 at 25°C |
Suspend 46.0 G of powder in 1 L of distilled or deionized water. If necessary, adjust the pH to 8.4 ± 0.2. Heat to boiling until completely dissolved. DO NOT AUTOCLAVE. Cool to 45 - 50°C in water bath. Aseptically add 2 vials of Tetrathionate Broth A supplement (MB-T0810) and add 2 vials of Tetrathionate Broth B supplement (MBT0757). Mix well. Pour into tubes. This complete medium should be used on the day of preparation
1 vial contents (each vial is sufficient for 500 mL of medium) | |
Iodine | 3.0 g |
Potassium Iodine | 2.5 g |
1 vial contents (each vial is sufficient for 500 mL of medium) | |
Brilliant Green | 0.005 g |
Tetrathionate Broth is a medium used for the isolation and cultivation of Salmonella spp. Polypeptone provides essential nutrients, growth factors and vitamins. Bile salts inhibits growth of Gram-positive bacteria. Calcium carbonate neutralizes the acidic tetrathionate decomposition products. The selectivity of the medium depends on the ability of thiosulfate and tetrathionate which suppress commensal coliforms. Organisms which possess tetrathionate reductase will grow in the medium.
Inoculate the specimen using a sterile needle to the medium. Incubate at 35 ± 2°C for 18 - 24 hours and then subculture onto MacConkey Agar and incubate plated media at 35 ± 2°C for 18 - 24 hours. Refer appropriate references for recommended test procedure.
Dehydrated medium
Appearance: free-flowing, homogeneous
Color: light beige, may have a slight greenish tint
Prepared medium
Appearance: supernatant over a heavy white precipitate
Color: light green
Incubation conditions: 35 ± 2 °C / 18 - 24 hours
then subculture onto MacConkey Agar / 35 ± 2 °C / 18 - 24 hours
Microorganism | ATCC | Inoculum CFU | Growth |
Salmonella typhimurium | 14028 | 50-100 | good |
Escherichia coli | 25922 | ≥10³ | partially inhibited |
The powder is very hygroscopic. Store the powder at room temperature, in a dry environment, in its original container tightly closed and use it before the expiry date on the label. Store prepared medium at 2 - 8°C.
1. The United States Pharmacopeia XXI (1985). Microbial Limit Tests. Rockville, Md.
2. American Public Health Association (1980). Standard methods for the examination of water and wastewater. 15th ed. APHA Inc. Washington DC.
3. Refer to the MFDS.
Cat. No : MB-T1183 Tetrathionate Broth | 500 G |
( 출처 : 기산바이오 )
상품견적 및 기타 상품관련 문의 게시판입니다.
★빠른 견적 이용방법★ 문의하고자 하시는 제품을 장바구니에 담으신 후 [견적서출력] 기능을 이용하시면, 빠르게 견적서를 출력하실 수 있습니다.
게시물이 없습니다