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상품명 | [MBCELL] UVM Modified Listeria Enrichment Broth (500g) (99336) |
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판매가 | ₩156,200 (VAT 포함) |
수량 | |
적립금 | 1,560원 |
★ 기타 | MBCELL 주문시 반품 및 교환 불가 (본사 지침사항) |
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네이버페이 구매 시에는 제주도지역 추가 배송비가 자동적용이 안되기 때문에 추가 배송비를 별도로 입금을 해주셔야 합니다. 기업은행 052-072431-01-014 예금주: ㈜비엔에프코리아, 추가배송비 3,000원 입금부탁드리며, 입금확인 후 출고진행되오니 이점 양해부탁드립니다. ★구매자명으로 입금해주세요~Medium used for the isolation and cultivation of Listeria spp.
*Equally use with MFDS (MB-U2193K) and QIA (MB-U2193Q).
CONTENTS (Liter) | Tryptose | 10.0 g |
Beef Extract | 5.0 g | |
Yeast Extract | 5.0 g | |
Sodium Chloride | 20.0 g | |
Disodium Phosphate | 9.6 g | |
Monopotassium Phosphate | 1.35 g | |
Esculin | 1.0 g | |
Nalidixic Acid | 0.02 g | |
Acriflavine HCl | 0.012 g | |
Final pH = 7.2 ± 0.2 at 25°C |
Suspend 51.98 G of powder in 1 L of distilled or deionized water. Heat until completely dissolved. Sterilize by autoclave at 121°C for 15 minutes. Cool to 45 - 50°C in water bath. Mix well. Pour into tubes.
UVM Modified Listeria Enrichment Broth is a medium used for the isolation and cultivation of Listeria spp. Tryptose, beef extract and yeast extract provide nitrogen, vitamins, and minerals. Sodium chloride maintains the osmotic balance. Phosphates are the buffering agents. Esculin is hydrolyzed by Listeria species. Nalidixic acid inhibits the growth of Gram-negative bacteria and acriflavin HCl is added to suppress the growth of Grampositive organisms.
Inoculate the specimen with stab using a sterile needle to the medium. Incubate at 30°C for 24 ± 2 hours. Refer appropriate references for recommended test procedure.
Dehydrated medium
Appearance: free-flowing, homogeneous
Color: beige
Prepared medium
Appearance: clear to hazy
Color: yellow
Incubation conditions: 30°C / 24 ± 2 hours
Microorganism | ATCC | Inoculum CFU | Growth |
Listeria monocytogenes | 15313 | 50-100 | good |
Staphylococcus aureus | 25923 | ≥10³ | partial to complete inhibition |
The powder is very hygroscopic. Store the powder at room temperature, in a dry environment, in its original container tightly closed and use it before the expiry date on the label or until signs of deterioration are evident. Store prepared medium at 2 - 8°C.
1. Kramer and Jones. 1969. J. Appl. Bacteriol. 32:381.
2. Donnelly and Baigent. 1986. Appl. Environ. Microbiol. 52:689.
3. Donnelly C.W. and Baigent G.J. (1986) Appl. Environ. Microbiol. 52. 689-695.
4. McClain D. and Lee W.H. (1988) Assoc. Off. Anal. Chem. 71. 660-664.
5. Bailey J.S., Fletcher D.L. and Cox N.A. (1990) J. Food Prot. 53. 473-477.
6. Refer to the MFDS, QIA
Cat. No : MB-U2193 UVM Modified Listeria Enrichment Broth | 500 G |
( 출처 : 기산바이오 )
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