스토어 정보 |
---|
상품명 | [MBCELL] Modified TSB (Tryptic Soy Broth) (500g) (99179) |
---|---|
판매가 | ₩264,000 (VAT 포함) |
수량 | |
적립금 | 2,640원 |
★ 기타 | MBCELL 주문시 반품 및 교환 불가 (본사 지침사항) |
구매방법 | |
---|---|
배송주기
정기배송 할인 save
|
상품 정보 | 가격 | 삭제 |
---|
할인가가 적용된 최종 결제예정금액은 주문 시 확인할 수 있습니다.
*** 네이버페이 구매 시 제주도 지역 추가 배송비 안내 ***
네이버페이 구매 시에는 제주도지역 추가 배송비가 자동적용이 안되기 때문에 추가 배송비를 별도로 입금을 해주셔야 합니다. 기업은행 052-072431-01-014 예금주: ㈜비엔에프코리아, 추가배송비 3,000원 입금부탁드리며, 입금확인 후 출고진행되오니 이점 양해부탁드립니다. ★구매자명으로 입금해주세요~Medium used for the isolation and cultivation of Enterohemorrhagic Escherichia coli O157:H7 in foods.
*Equally use with MFDS (MB-T0789K).
CONTENTS (Liter) | Peptone from Casein | 17.0 g |
Peptone from Soymeal | 3.0 g | |
Sodium Chloride | 1.5 g | |
Bile Salts No.3 | 2.5 g | |
D(+)-Glucose | 2.5 g | |
Dipotassium Phosphate | 4.0 g | |
Final pH = 7.3 ± 0.2 at 25°C |
Suspend 30.5 G of powder in 1 L of distilled or deionized water. Heat to boiling until completely dissolved. Sterilize by autoclave at 121°C for 15 minutes. Cool to 45 - 50°C in water bath. Aseptically add 2 vials of Novobiocin supplement (MB-N1821). Mix well. Pour into tubes.
1 vial contents (each vial is sufficient for 500 mL of medium) | |
Novobiocin | 0.01 g |
TSB (Tryptic Soy Broth) Modified is a medium used for the isolation and cultivation of Enterohemorrhagic Escherichia coli O157:H7 in foods. Peptone from casein, peptone from soymeal provide nitrogen, carbon, vitamins and minerals. Sodium chloride maintains the osmotic balance. Bile salts No.3 and novobiocin supplement inhibits the growth of Gram-positive bacteria. D(+)-Glucose is the carbohydrate. Dipotassium phosphate is the buffering agent.
Inoculate the specimen using a sterile needle to the medium. Incubate at 35 ± 2°C for 18 - 24 hours. Refer appropriate references for recommended test procedure.
Dehydrated medium
Appearance: free-flowing, homogeneous
Color: light beige
Prepared medium
Appearance: clear
Color: light amber
Incubation conditions: 35 ± 2°C / 18 - 24 hours
Microorganism | ATCC | Inoculum CFU | Growth |
Escherichia coli O157:H7 | 35150 | 50-100 | good |
Escherichia coli | 25922 | 50-100 | good |
Enterococcus faecalis | 29212 | ≥10³ | partially inhibited |
Staphylococcus aureus | 25923 | ≥10³ | partially inhibited |
The powder is very hygroscopic. Store the powder at room temperature, in a dry environment, in its original container tightly closed and use it before the expiry date on the label. Store prepared medium at 2 - 8°C.
1. U.S. FDA. Center for Food Safety & Applied Nutrition. 2001. Food pathogenic microorganisms and natural toxins handbook. Escherichia coli O157:H7. College Park, MD.
2. Hill, W.E., A. R. Datta, P. Feng, K. A. Lampel, and W. L. Payne. 1998. FDA Bacteriological analytical manual, 8th ed. Identification of Foodborne Bacterial Pathogens by Gene Probes. AOAC International, Gaithersburg, MD.
3. DIN Deutsches Institut für Normung e.V., Nachweis von Escherichia coli 0157 in Lebensmitteln, DIN 10167.
4. FDA Bacteriological Analytical Manual, 8th Edition/1995, Chapter 4. Escherichia coli and the Coliform Bacteria, page 4.20: Isolation Methods for Enterohemorrhagic E. coli (EHEC).
5. S.D. Weagant, J.L. Bryant, K.G. Jinneman, An improved rapid technique for isolation of Escherichia coli 0157:H7 from foods, J. Food Prot., 58; 7-12 (1995)
6. Refer to the MFDS.
Cat. No : MB-T0789 TSB (Tryptic Soy Broth) Modified | 500 G |
( 출처 : 기산바이오 )
상품견적 및 기타 상품관련 문의 게시판입니다.
★빠른 견적 이용방법★ 문의하고자 하시는 제품을 장바구니에 담으신 후 [견적서출력] 기능을 이용하시면, 빠르게 견적서를 출력하실 수 있습니다.
게시물이 없습니다